Refine your search
Collections
Co-Authors
Journals
Year
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Vaishnav, Anukool
- Comparative Expression Analysis of Defence-Related Genes in Bacillus-Treated Glycine Max upon Challenge Inoculation with Selective Fungal Phytopathogens
Abstract Views :247 |
PDF Views:71
Authors
Affiliations
1 Mandsaur University, Mandsaur 458 001, IN
2 Amity Institute of Microbial Technology, Amity University, Noida 201 303,, IN
1 Mandsaur University, Mandsaur 458 001, IN
2 Amity Institute of Microbial Technology, Amity University, Noida 201 303,, IN
Source
Current Science, Vol 115, No 10 (2018), Pagination: 1950-1956Abstract
Activation of defence-related genes by the application of beneficial bacteria leads to prior protection against pathogens through induced systemic resistance. The present study was carried out to examine the qRT– PCR-based relative quantification of differently expressed defence-related genes in soybean (Glycine max L. Merrill) plants primed with Bacillus sp. strain SJ-5 against the fungal pathogen Rhizoctonia solani and Fusarium oxysporum. In this context, molecular characterization of plant growth promoting and biocontrol genes of SJ-5 was done by PCR followed by homology analysis. In the GC-MS analysis of SJ-5 volatile organic compounds, potent antifungal compound bis(2-ethyl-hexyl) phthalate and antioxidant compound butylated hydroxy toluene were reported with the highest peak area 47.96% and 21.82% respectively, along with other antifungal compounds in small proportion. Qualitative expression of different defence-related genes like lipoxygenase, phenylalanine ammonia-lyase 2, peroxidase, polyphenol oxidase, endo-1,3-beta-glucanase, catalase, defensin-like protein, vegetative storage protein and chitinase class I was found elicited in the plants primed with SJ-5 against the fungal pathogens. In the qPCR analysis, the highest upregulation was observed in the transcript profile of ppojh2 in the treatments T5 and T6 with 4.12- and 4.06-fold increase respectively.Keywords
Defence-Related Genes, Induced Systemic Resistance, Plant Growth Promoting Bacteria, Volatile Organic Compounds.Full Text
References
- Hashem, E. A., Abdalla, H. E., Hussein, Y. A. and Abd-Elnabi, M. A., In vitro selection of soybean callus resistant to Fusarium oxysporum metabolites. Res. J. Agric. Biol. Sci., 2009, 5, 588–596.
- Jain, S., Varma, A., Tuteja, N. and Choudhary, D. K., Plant growth promoting microbial-mediated induced systemic resistance in plants: induction, mechanism and expression. In MicrobialMediated Induced Systemic Resistance in Plants (eds Choudhary, D. K. and Varma, A.), Springer, Singapore, 2016, pp. 213–226.
- Pieterse, C. M. J., Leon-Reyes, A., Van der Ent, S. and Van Wees, S. C. M., Networking by small-molecule hormones in plant immunity. Nature Chem. Biol., 2009, 5, 308–316.
- Yang, Y. X., Ahammed, G. J., Wu, C., Fan, S. Y. and Zhou, Y. H., Crosstalk among jasmonate, salicylate and ethylene signaling pathways in plant disease and immune responses. Curr. Protein Pept. Sci., 2015, 16, 450–461.
- McConn, M., Creelman, R. A., Bell, E., Mullet, J. E. and Browse, J., Jasmonate is essential for insect defense in Arabidopsis. Proc. Natl. Acad. Sci. USA, 1997, 94, 5473–5477.
- Mauch, F., Mauch-Mani, B. and Boller, T. Antifungal hydrolases in pea tissue. II. Inhibition of fungal growth by combinations of chitinases and b-1, 3-glucanase. Plant Physiol., 1988, 88, 936–942.
- Ryu, C. M., Hu, C. H., Reddy, M. S. and Kloepper, J. W., Different signaling pathways of induced resistance by rhizobacteria in Arabidopsis thaliana against two pathovars of Pseudomonas syringae. New Phytol., 2003, 160, 413–420.
- Choudhary, D. K., Sharma, K. P. and Gaur, R. K., Biotechnological perspectives of microbes in agro-ecosystems. Biotechnol. Lett., 2011, 33, 1905–1910.
- Bollet, C., Gevaudan, M. J., de Lamballerie, X., Zandotti, C. and de Micco, P., A simple method for the isolation of chromosomal DNA from Gram positive or acid fast bacteria. Nucleic Acids Res., 1991, 19, 1955.
- Tamura, K., Stecher, G., Peterson, D., Filipski, A. and Kumar, S., MEGA6: molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol., 2013, 30, 2725–2729.
- Jain, S., Vaishnav, A., Kumari, S., Varma, A., Tuteja, N. and Choudhary, D. K., Chitinolytic Bacillus-mediated induction of jasmonic acid and defense-related proteins in soybean (Glycine max L. Merrill) plant against Rhizoctonia solani and Fusarium oxysporum. J. Plant Growth. Regul., 2017, 36, 200–214.
- Deubel, A., Gransee, A. and Merbach, W., Transformation of organic rhizodeposits by rhizoplane bacteria and its influence on the availability of tertiary calcium phosphate. J. Plant Nutr. Soil. Sci., 2000, 163, 387–392.
- de Werra, P., Péchy-Tarr, M., Keel, C. and Maurhofer, M., Role of gluconic acid production in the regulation of biocontrol traits of Pseudomonas fluorescens CHA0. Appl. Environ. Microbiol., 2009, 75, 4162–4174.
- Spaepen, S., Vanderleyden, J. and Remans, R., Indole-3-acetic acid in microbial and microorganism–plant signaling. FEMS Microbiol. Rev., 2007, 31, 425–448.
- Sun, Y., Cheng, Z. and Glick, B. R., The presence of a 1-aminocyclopropane1-carboxylate (ACC) deaminase deletion mutation alters the physiology of the endophytic plant growth-promoting bacterium Burkholderia phytofirmans PsJN. FEMS Microbiol. Lett., 2009, 296, 131–136.
- Xu, T., Zhu, T. and Li, S., β-1, 3-1, 4-glucanase gene from Bacillus velezensis ZJ20 exerts antifungal effect on plant pathogenic fungi. World J. Microbiol. Biotechnol., 2016, 32, 1–9.
- Yang, C. Y., Ho, Y. C., Pang, J. C., Huang, S. S. and Tschen, J. S., Cloning and expression of an antifungal chitinase gene of a novel Bacillus subtilis isolate from Taiwan potato field. Bioresour. Technol., 2009, 100, 1454–1458.
- Beligni, M. V., Fath, A., Bethke, P. C., Lamattina, L. and Jones, R. L., Nitric oxide acts as an antioxidant and delays programmed cell death in barley aleurone layers. Plant Physiol., 2002, 129, 1642–1650.
- Kavitha, A., Prabhakar, P., Vijayalakshmi, M. and Venkateswarlu, Y., Production of bioactive metabolites by Nocardia levis MK-VL_113. Lett. Appl. Microbiol., 2009, 49, 484–490.
- Al-Bari, M. A., Rahman, M. S., Islam, M. A., Flores, M. E. and Bhuiyan, M. S., Purification and characterization of a β-(1,4)-endoxylanase of Streptomyces bangladeshiensis sp. Res. J. Cell. Mol. Biol., 2007, 1, 31–36.
- Hoang, V. L., Li, Y. and Kim, S. K., Cathepsin B inhibitory activities of phthalates isolated from a marine Pseudomonas strain. Bioorg. Med. Chem. Lett., 2008, 18, 2083–2088.
- Namikoshi, M., Fujiwara, T., Nishikawa, T. and Ukai, K., Natural abundance 14C content of dibutyl phthalate (DBP) from three marine algae. Mar. Drugs, 2006, 4, 290–297.
- Amade, P., Mallea, M. and Bouaicha, N., Isolation, structural identification and biological activity of two metabolites produced by Penicillium olsonii Bainier and Sartory. J. Antibiot., 1994, 47, 201–207.
- Ahsan, T., Chen, J., Zhao, X., Irfan, M. and Wu, Y., Extraction and identification of bioactive compounds (Eicosane and dibutyl phthalate) produced by Streptomyces strain KX852460 for the biological control of Rhizoctonia solani AG-3 strain KX852461 to control target spot disease in tobacco leaf. AMB Exp., 2017, 7, 54.
- Kether, F. B., Mahjoub, M. A., Mahjoub, S. A., Salah, K. B., Helal, A. N. and Mighri, Z., Chemical composition, in vitro antifungal and antioxidant activities of essential oil from Cotula coronopifolia L. growing in Tunisia. Afr. J. Microbiol. Res., 2012, 6, 4388–4395.
- Pohl, C. H., Kock, J. L. and Thibane, V. S., Antifungal free fatty acids: a review. In Science against Microbial Pathogens: Current Research and Technological Advances (ed. Mendez-Vilas, A.), FORMATEX Research Center, Spain, 2011, pp. 61–71.
- Barrera-Necha, L. L., Garduno-Pizana, C. and Garcia-Barrera, L. J., In vitro antifungal activity of essential oils and their compounds on mycelial growth of Fusarium oxysporum f. sp. gladioli (Massey) Snyder and Hansen. Plant Pathol. J., 2009, 8, 17–21.
- Desmond, O. J., Edgar, C. I., Manners, J. M., Maclean, D. J., Schenk, P. M. and Kazan, K., Methyl jasmonate induced gene expression in wheat delays symptom development by the crown rot pathogen Fusarium pseudograminearum. Physiol. Mol. Plant Pathol., 2006, 67, 171–179.
- Ebadzad, G. and Cravador, A., Quantitative RT-PCR analysis of differentially expressed genes in Quercus suber in response to Phytophthora cinnamomi infection. Springerplus, 2014, 3, 1–13.
- Dos Santos, C. V., Letousey, P., Delavault, P. and Thalouarn, P., Defense gene expression analysis of Arabidopsis thaliana parasitized by Orobanche ramosa. Phytopathology, 2003, 93, 451–457.